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Response of a murine V beta 18-alloreactive CTL clone to bacterial superantigens
L A Hwang1, T K Sabapathy, K M Hui
1Laboratory of Molecular Immunology, National University of Singapore.
Abstract:
We have isolated, by limiting dilution, a murine anti-H-2Kb T cell line derived from AKR/J mouse spleen cells following stimulation with H-2Kb+ cells. This clone, designated 2AH10, has the phenotype H-2Kk+, CD8+, LFA-1+, ICAM-1+, and IL-2R+ and, as determined by DNA-sequencing reactions, expresses the V beta 18 T cell receptor gene segment. Clone 2AH10 specifically lysed H-2Kb+ target cells and on stimulation with H-2Kb+ cells proliferated to secrete IFN-gamma, LT, and GM-CSF. Clone 2AH10 was responsive to the bacterial superantigens SEC1 and TSST-1 when stimulated in the context of murine MHC class II+ cells. In addition to SEC1 and TSST-1, when human MHC class II+ cells were employed, clone 2AH10 responded to a wider range of bacterial superantigens including SEB, SED, and SEE.