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Cloning of cDNA for human granzyme 3
M M Przetak1, S Yoast, B F Schmidt
1Khepri Pharmaceuticals Inc., South San Francisco, CA 94080, USA.
FEBS Letters
|May 15, 1995
Summary
Researchers identified a new human serine protease, granzyme 3, crucial for immune responses. This gene is highly expressed in key immune tissues, suggesting its significant role in cellular immunity.
Area of Science:
- Biochemistry
- Immunology
- Molecular Biology
Background:
- Serine proteases play vital roles in various biological processes, including immune responses.
- Human granzymes are a family of serine proteases known for their cytotoxic functions.
Purpose of the Study:
- To clone and characterize a novel serine protease gene from human ascites tissue.
- To identify and analyze the features of the encoded protein, designated as human granzyme 3.
Main Methods:
- cDNA cloning from human ascites tissue.
- N-terminal amino acid sequencing for protein identification.
- Northern blot analysis for gene expression profiling.
Main Results:
- A serine protease gene encoding a 264-amino acid protein, human granzyme 3, was successfully cloned.
- Human granzyme 3 exhibits characteristics of the chymotrypsin family and shows high identity (75%) to rat tryptase RNK-Tryp-2.
- High expression of granzyme 3 was observed in peripheral blood leukocytes, spleen, thymus, and lung tissues.
Conclusions:
- Human granzyme 3 is a newly identified serine protease with potential roles in the immune system.
- Its expression pattern suggests involvement in immune cell function and inflammatory responses.