Related Experiment Video

Updated: Aug 18, 2026

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis
16:01

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

Published on: January 26, 2015

[Morphological and functional status of mast cells in the late stage of acute infectious inflammation]

S V Tatarko, M O Klymenko

    Fiziolohichnyi Zhurnal (Kiev, Ukraine : 1994)
    |March 1, 1994
    PubMed

    Insights

    Mast cells repopulate and degranulate during inflammation repair in rats. Their activity, particularly histamine release, suggests a role in the late stages of healing after acute E. coli peritonitis.

    Area of Science:

    • Immunology
    • Pathology

    Background:

    • Acute inflammation involves complex cellular responses.
    • Mast cells are key players in inflammatory and reparative processes.

    Purpose of the Study:

    • To investigate mast cell behavior during the late reparative stage of acute E. coli-induced infectious peritonitis in rats.
    • To assess the role of mast cells and histamine in inflammation resolution.

    Main Methods:

    • Utilized a rat model of acute E. coli-induced infectious peritonitis.
    • Monitored mast cell numbers and degranulation in peritoneal fluid and mesentery over 120 days.
    • Measured free histamine content and blood histamine concentration.

    Main Results:

    • Mast cell repopulation and degranulation occurred in the late reparative phase.
    • Mast cell numbers recovered by day 90, but peritoneal mast cell regranulation lagged behind mesenteric cells.
    • Increased mast cell degranulation and histamine levels indicated active involvement in late reparative events.

    Conclusions:

    • Mast cells likely play an active role in the late reparative stages of acute inflammation.
    • Histamine release from mast cells may contribute to tissue repair following peritonitis.

    More Related Videos

    Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice09:07

    Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice

    Published on: May 27, 2015

    Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury14:48

    Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury

    Published on: March 21, 2021

    Related Concept Videos

    Inflammation01:38

    Inflammation

    Overview
    Differentiation of Common Myeloid Progenitor Cells01:15

    Differentiation of Common Myeloid Progenitor Cells

    Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
    Inflammatory Response I: Vascular and Cellular01:30

    Inflammatory Response I: Vascular and Cellular

    The inflammatory response is the body's defense against infection, injury, or irritation from bacteria, trauma, toxins, or heat. Inflammation helps locate and destroy pathogens and remove damaged tissue elements to heal the body. During this initial phase, fluid, blood products, and nutrients migrate to the injured area, resulting in redness, heat, swelling, ache, and loss of function. Moreover, signs of systemic inflammation include fever, increased WBC count, malaise, anorexia, nausea,...
    Acute Inflammation I: Inflammatory Response01:26

    Acute Inflammation I: Inflammatory Response

    Acute inflammation is a rapid, short-lived physiological response to tissue injury or infection, designed to eliminate harmful agents and initiate repair. This tightly regulated process typically lasts from minutes to several days and is triggered by factors such as microbial invasion, physical trauma, or chemical injury.Recognition and Mediator ReleaseThe inflammatory response begins when resident immune cells—such as mast cells, macrophages, and dendritic cells—detect damage-associated...
    Acute Inflammation II: Cellular Phase01:26

    Acute Inflammation II: Cellular Phase

    The cellular phase of acute inflammation is a tightly orchestrated sequence of events that recruits leukocytes, primarily neutrophils, to sites of tissue injury or infection. Following the initial vascular changes, this phase ensures effective immune cell migration, activation, and function at the affected site to eliminate pathogens and initiate tissue repair.Leukocyte Recruitment CascadeLeukocyte recruitment happens in four steps: margination, adhesion, transmigration, and chemotaxis. Reduced...
    Chronic Inflammation: Introduction01:12

    Chronic Inflammation: Introduction

    Chronic inflammation is a prolonged, dysregulated immune response that persists for weeks to years when the inciting stimulus is difficult to eradicate or when self‑antigens drive ongoing reactivity. Morphologically, it is defined by mononuclear cell infiltration, progressive tissue destruction, and concurrent attempts at healing via angiogenesis and fibrosis. Compared with acute inflammation, edema is less prominent while cellular infiltration predominates; triggers include persistent...