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Related Experiment Videos

Coding end processing is similar throughout ontogeny

B Nadel1, S Tehranchi, A J Feeney

  • 1Department of Immunology, Scripps Research Institute, La Jolla, CA 92037, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|June 15, 1995
PubMed
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Newborn B and T cell diversity is limited by a lack of terminal deoxynucleotidyltransferase (TdT). This study reveals consistent nucleotide deletion during V(D)J recombination across development, independent of TdT presence.

Area of Science:

  • Immunology
  • Molecular Biology
  • Developmental Biology

Background:

  • V(D)J recombination generates diverse B and T cell receptors.
  • Newborn immune cell diversity is significantly lower than adult diversity.
  • Terminal deoxynucleotidyltransferase (TdT) absence in newborns limits N-region addition, a key source of diversity.

Purpose of the Study:

  • To investigate coding end processing during V(D)J recombination throughout ontogeny.
  • To clarify the role of TdT and homology-directed recombination in shaping immune receptor diversity.
  • To analyze nucleotide deletion patterns at coding ends in the presence and absence of TdT.

Main Methods:

  • Comparative analysis of coding end processing in normal and TdT-deficient mice.
  • Focus on partial D-J joints to minimize selection bias.

Related Experiment Videos

  • Assessment of nucleotide deletion extent and processing patterns throughout ontogeny.

    • Main Results:

    • Nucleotide deletion at coding ends is consistent across ontogeny, irrespective of TdT expression.
    • Homology-directed recombination, prevalent early in life, can obscure coding end processing analysis.
    • Distinct processing patterns were identified for individual coding ends.

    Conclusions:

    • The extent of nucleotide deletion during V(D)J recombination is largely conserved throughout development.
    • TdT absence primarily affects N-region addition, not the fundamental deletion process at coding ends.
    • Understanding these processing mechanisms is crucial for comprehending immune repertoire development and diversity.