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Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
Published on: September 16, 2011
Identification and partial characterization of a unique marker for human basophils
C L Kepley1, S S Craig, L B Schwartz
1Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond 23298, USA.
Journal of Immunology (Baltimore, Md. : 1950)
|June 15, 1995
Summary
Researchers identified a novel marker, 2D7, specific to human basophils. This antibody targets secretory granules, aiding in the study of basophil involvement in allergic diseases.
Area of Science:
- Immunology
- Cell Biology
Background:
- Human basophils play a role in clinical disease, but a specific marker is needed for precise assessment.
- Identifying a marker for basophil secretory granules is crucial for understanding their function.
Purpose of the Study:
- To identify and characterize a unique marker for human basophils.
- To investigate the localization and potential use of this marker in disease assessment.
Main Methods:
- Generation of mouse hybridomas against human basophils.
- Screening for basophil-selective antibodies using immunoperoxidase and immunogold electron microscopy.
- Western blotting to determine the molecular weight of the target ligand.
Main Results:
- A novel antibody, 2D7 (IgG1 kappa), was identified that specifically labels human basophils.
- The 2D7 ligand is localized to secretory granules and its staining intensity decreases upon basophil activation.
- Western blotting revealed two predominant bands at 76,150 and 72,260 Da.
Conclusions:
- The 2D7 antibody serves as a specific marker for human basophils.
- This marker can facilitate the assessment of basophil involvement in diseases like asthma and atopic dermatitis.
