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Related Experiment Videos

Connective tissue: a metabolic entity?

K T Weber1, Y Sun, L C Katwa

  • 1Department of Internal Medicine, University of Missouri Health Sciences Center, Columbia, USA.

Journal of Molecular and Cellular Cardiology
|January 1, 1995
PubMed
Summary

The heart's connective tissue is metabolically active, regulating peptide hormones that control collagen turnover. This challenges the view of stroma as inert, revealing its dynamic role in cardiac health.

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Area of Science:

  • Cardiovascular Biology
  • Connective Tissue Research
  • Molecular Cardiology

Background:

  • The heart comprises parenchyma (cardiac myocytes) and stroma (connective tissue).
  • Cardiac stroma has been considered metabolically inert, receiving limited research attention.
  • Emerging evidence suggests connective tissue plays a more active regulatory role.

Purpose of the Study:

  • To investigate the metabolic activity of cardiac connective tissue.
  • To explore the role of stroma in regulating peptide hormone generation and degradation.
  • To determine if these hormones influence collagen turnover in an autocrine manner.

Main Methods:

  • Quantitative in vitro autoradiography using 125I-351A to localize angiotensin-converting enzyme (ACE) binding density.

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  • Immunohistochemistry with monoclonal ACE antibody.
  • In situ hybridization to detect type I collagen transcripts.
  • Receptor-ligand binding assays for angiotensin II and bradykinin.

    • Main Results:

    • ACE binding density is heterogeneous in the heart, with high concentrations in areas of high collagen turnover (valve leaflets, adventitia, fibrous tissue).
    • ACE activity in these sites is independent of circulating angiotensin II.
    • Fibroblast-like cells expressing alpha-actin and type I collagen transcripts produce ACE.
    • Angiotensin II and bradykinin receptors are present in fibrous tissue, suggesting autocrine regulation of collagen turnover.
    • ACE inhibition or angiotensin II receptor antagonism attenuates connective tissue formation.

    Conclusions:

    • Cardiac stroma is a dynamic, metabolically active entity, not inert.
    • Connective tissue regulates its own peptide hormone composition.
    • This regulation influences the turnover of fibrillar collagen in the heart.