Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An efficient method to isolate yeast genes causing overexpression-mediated growth arrest

C Espinet1, M A de la Torre, M Aldea

  • 1Departament de Ciències Mèdiques Bàsiques, Facultat de Medicina, Universitat de Lleida, Spain.

Yeast (Chichester, England)
|January 1, 1995
PubMed
Summary

Researchers identified new yeast genes controlling cell proliferation by isolating overexpression-sensitive clones. Overexpressing MCM1 and other new genes caused cell cycle arrest, revealing novel regulators of yeast growth.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sex-based differences in tolerability of developmental antibody-drug conjugates (ADCs) in non-small-cell lung cancer (NSCLC).

ESMO open·2026
Same author

Lung cancer brain metastases management at the dawn of personalized medicine: are we ready to break the barriers?

Annals of oncology : official journal of the European Society for Medical Oncology·2026
Same author

MTAP loss is frequent in oncogene-driven NSCLC and may confer sensitivity to combined PRMT5 inhibitors and targeted therapies.

Annals of oncology : official journal of the European Society for Medical Oncology·2026
Same author

Next-generation multicenter studies: using artificial intelligence to automatically process unstructured health records of patients with lung cancer across multiple institutions.

Annals of oncology : official journal of the European Society for Medical Oncology·2025
Same author

ESMO basic requirements for AI-based biomarkers in oncology (EBAI).

Annals of oncology : official journal of the European Society for Medical Oncology·2025
Same author

Immunotherapy beyond progression combined with platinum-based chemotherapy after primary resistance to first-line immunotherapy in patients with advanced NSCLC and PD-L1 ≥50.

ESMO open·2025

Area of Science:

  • Molecular and Cellular Biology
  • Yeast Genetics
  • Cell Cycle Regulation

Background:

  • Understanding yeast cell proliferation is crucial for fundamental biology and biotechnology.
  • Identifying genes that regulate cell division is key to understanding growth control.

Purpose of the Study:

  • To identify novel Saccharomyces cerevisiae genes that regulate cell proliferation.
  • To characterize the phenotype of overexpression-sensitive yeast clones.

Main Methods:

  • Screening of a Saccharomyces cerevisiae cDNA library for overexpression-sensitive clones.
  • Utilizing the GAL1 promoter for galactose-inducible gene expression.
  • Analyzing cell cycle progression and morphology upon gene overexpression.

Related Experiment Videos

Main Results:

  • Isolated 11 novel yeast genes with unknown functions regulating cell proliferation.
  • Confirmed 13 known genes involved in morphogenesis and transcriptional regulation.
  • Overexpression of MCM1 and other identified genes induced cell cycle arrest at G1 or S phases.

Conclusions:

  • The study identified new yeast genes that play critical roles in cell proliferation control.
  • Overexpression of these genes leads to cell cycle arrest, highlighting their regulatory functions.
  • The findings expand the repertoire of known yeast genes influencing cell division.