Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA preparation from cryopreserved bone marrow cell samples

H Jørgensen1, P Hokland, E L Petersen

  • 1Institute of Medical Microbiology, University of Aarhus, Denmark.

Analytical Biochemistry
|March 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An experimental apparatus for the study of high-temperature degradation and solid-deposit formation of lubricants.

The Review of scientific instruments·2024
Same author

Influence of dietary fibre on nutrient digestibility and energy utilisation in growing pigs fed diets varying in soluble and insoluble fibres from co-products.

Animal : an international journal of animal bioscience·2022
Same author

Digestibility of fractionated green biomass as protein source for monogastric animals.

Animal : an international journal of animal bioscience·2019
Same author

Chronic maternal inflammation or high-fat-feeding programs offspring obesity in a sex-dependent manner.

International journal of obesity (2005)·2017
Same author

Differential expression levels and methylation status of ROBO1 in mantle cell lymphoma and chronic lymphocytic leukaemia.

International journal of laboratory hematology·2016
Same author

Standardized ileal digestibility of amino acids in eight genotypes of barley fed to growing pigs.

Animal : an international journal of animal bioscience·2016
Same journal

Lysozyme assay using a rationally designed GN4G2 substrate with coupled β-glucosidase reaction.

Analytical biochemistry·2026
Same journal

The long run: A tribute to Arthur Joseph Lawrence Cooper.

Analytical biochemistry·2026
Same journal

Evaluation of a method for affinity measurement using solution equilibrium titration with magnetic beads.

Analytical biochemistry·2026
Same journal

Metabolomics approach using UHPLC/QE-MS for the mechanism of He Xue Ming Mu tablets on non-proliferative diabetic retinopathy.

Analytical biochemistry·2026
Same journal

UniRES-GO: Unified residue-level early fusion of sequence and predicted structure for protein function prediction.

Analytical biochemistry·2026
Same journal

IgG detection by enzyme-linked mass spectrometric assay versus color, fluorescent, ECL in buffer and serum.

Analytical biochemistry·2026
See all related articles

This study presents a DNA extraction method for cryopreserved cells, enhancing viability using DNase I and immunomagnetic bead separation. This technique yields high-quality DNA from low-viability samples, crucial for hematological research.

Area of Science:

  • Hematology
  • Molecular Biology
  • Cell Biology

Background:

  • Cryopreserved samples often have low cell viability.
  • Standard DNA extraction methods may be insufficient for compromised samples.
  • High-quality DNA is essential for accurate molecular analyses in hematological research.

Purpose of the Study:

  • To develop an optimized DNA extraction protocol for cryopreserved cells with low initial viability.
  • To improve cell viability prior to DNA extraction.
  • To enable reliable DNA analysis from challenging sample sources.

Main Methods:

  • Cryopreserved cells were pretreated with DNase I to enhance viability.
  • Immunomagnetic bead separation was employed to isolate viable cells.
  • DNA was extracted and its quality/yield assessed by spectrophotometry.

Related Experiment Videos

Main Results:

  • The method achieved near 100% cell viability post-treatment.
  • High-quality DNA was successfully extracted from cryopreserved samples.
  • The protocol allows for DNA analysis from selected cellular subsets.

Conclusions:

  • The described DNA extraction method effectively recovers high-quality DNA from cryopreserved cells, even those with low viability.
  • This technique is recommended for DNA analysis in hematological research using compromised bone marrow samples.
  • The method facilitates targeted DNA analysis on specific cell populations.