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Immunohistochemical study of mammary and extramammary Paget's disease
G Nakamura1, N Shikata, T Shoji
1Department of Pathology, Kansai Medical University, Osaka, Japan.
Abstract:
Immunophenotypes of mammary (MPD) and extramammary Paget's disease (EPD) are still not well understood. Thirty-four formalin-fixed paraffin-embedded tissue sections from 33 patients with 6 MPD and 28 EPD were studied immunohistochemically with the use of polyclonal c-erbB-2 and pS2 antisera, and monoclonal nm23, B6.2, GCDFP-15, and p53 antibodies. Cases of MPD expressed a high incidence of c-erbB-2 and nm23 compared with those of EPD (100% vs. 29%; p < 0.01, and 83% vs. 29%; p < 0.05, respectively). Although high expression of B6.2 (> 83%) and moderate expression of GCDFP-15 (33-39%), pS2 (33-46%) and p53 (39-50%) were seen, the positivity was not significantly different between MPD and EPD. These findings indicate that MPD and EPD share immunohistochemical features but partially differ in their patterns of antigen expression.
Insights
Mammary (MPD) and extramammary Paget's disease (EPD) share some immunophenotypes but differ in antigen expression. MPD shows higher c-erbB-2 and nm23 incidence than EPD, indicating distinct cellular characteristics.
Area of Science:
- Oncology
- Pathology
- Immunohistochemistry
Background:
- The immunophenotypes of mammary (MPD) and extramammary Paget's disease (EPD) require further elucidation.
- Understanding these distinctions is crucial for accurate diagnosis and targeted therapy.
Purpose of the Study:
- To investigate and compare the immunohistochemical profiles of MPD and EPD.
- To identify specific antigen expression patterns that differentiate between MPD and EPD.
Main Methods:
- Utilized immunohistochemistry on 34 formalin-fixed paraffin-embedded tissue sections from 33 patients (6 MPD, 28 EPD).
- Tested antibodies against c-erbB-2, pS2, nm23, B6.2, GCDFP-15, and p53.
Main Results:
- Mammary Paget's disease (MPD) exhibited significantly higher c-erbB-2 (100% vs. 29%) and nm23 (83% vs. 29%) expression compared to extramammary Paget's disease (EPD).
- While B6.2, GCDFP-15, pS2, and p53 showed varying expression levels, differences between MPD and EPD were not statistically significant for these markers.
Conclusions:
- Mammary (MPD) and extramammary Paget's disease (EPD) share common immunohistochemical features.
- Distinct patterns of antigen expression, particularly for c-erbB-2 and nm23, suggest partially different cellular origins or characteristics between MPD and EPD.