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Towards a molecular description of pulsed-field gel electrophoresis
C Bustamante1, S Gurrieri, S B Smith
1Institute of Molecular Biology, University of Oregon, Eugene 97403.
Trends in Biotechnology
|January 1, 1993
Abstract:
An improved understanding of the molecular processes of migration and reorientation of DNA molecules undergoing conventional and pulsed-field gel electrophoresis (PFGE) should provide the basis for the rational optimization of these techniques. This review of common pulsed-field methods emphasizes the range of their applications as well as their current limitations. The molecular mechanisms involved in electrophoresis, as they are beginning to emerge from a combination of direct observations using a microscope and computer-modeling studies, are also discussed.