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Related Concept Videos

Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

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Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
7.1K
Electrophoresis: Overview01:20

Electrophoresis: Overview

3.3K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
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SDS-PAGE01:27

SDS-PAGE

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Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
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DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

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Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
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Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
919
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

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Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
717

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Related Experiment Video

Updated: Dec 19, 2025

Author Spotlight: Streamlining Protein Target Prediction and Validation via Molecular Docking and CETSA
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Author Spotlight: Streamlining Protein Target Prediction and Validation via Molecular Docking and CETSA

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Protein electrophoresis

T E Zewert1, M G Harrington

  • 1Department of Biology, California Institute of Technology, Pasadena 91125.

Current Opinion in Biotechnology
|February 1, 1993
PubMed
Summary

Recent protein electrophoresis technologies have advanced significantly. Innovations in capillary and gel electrophoresis, along with blotting techniques, enhance protein analysis capabilities.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Protein electrophoresis is a cornerstone technique in biological research.
  • Continuous innovation is crucial for improving protein separation and detection.

Purpose of the Study:

  • To review recent advancements in protein electrophoresis and related technologies.
  • To highlight key improvements in capillary electrophoresis, blotting, and gel electrophoresis.

Main Methods:

  • Review of recent literature on protein electrophoresis techniques.
  • Analysis of innovations in apparatus design, detection methods, and material modifications.

Main Results:

  • Significant improvements in capillary electrophoresis, including apparatus, detection, and capillary modifications.

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  • Advances in protein blotting techniques for transfer, analysis, and removal.
  • Enhancements in gel electrophoresis through new hardware, protocols, and matrices.

    • Conclusions:

    • Recent technological progress has substantially improved protein electrophoresis methods.
    • These advancements offer enhanced capabilities for protein separation, detection, and analysis.