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Protoplast formation and regeneration in Streptomyces flavopersicus
D Lyutzkanova1, B Nikolova, M Stoilova-Discheva
1Institute of Microbiology, Bulgarian Academy of Sciences, Sofia.
Letters in Applied Microbiology
|April 1, 1993
Abstract:
Protoplasts of Streptomyces flavopersicus with the highest regeneration frequency were isolated from late log phase mycelium grown in a two-stage culture system with 2% glycine in the medium. Of the 11 regeneration media tested, R9 was selected as the most efficient with 29 degrees C as the best temperature.