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Separation of biomolecules using supercritical fluid extraction
F P Lucien1, K K Liong, N J Cotton
1School of Chemical Engineering and Industrial Chemistry, University of New South Wales, Kensington, Australia.
Australasian Biotechnology
|May 1, 1993
Abstract:
Supercritical fluids are increasingly being used as a replacement for more conventional organic solvents in the extraction of biomolecules from a range of matrices. Supercritical fluid extraction of essential fatty acids from fish oils is discussed. Supercritical CO2 was used to fractionate two fatty acids, EPA and DHA from fish oil ethyl esters. EPA and DHA were obtained with a purity of 58% and 67% respectively from Sardine oil with an original composition of 17% and 12%.