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A combined polymerase chain reaction-colour development hybridization assay in a microtitre format for the detection
I Galindo1, R Rangel-Aldao, J L Ramírez
1Gerencia Nacional de Biotecnología, Caracas, Venezuela.
Applied Microbiology and Biotechnology
|July 1, 1993
Abstract:
We have developed a rapid and sensitive assay for the detection of clostridial cells or spores in liquid food samples. The method recognizes Clostridium 16S (small ribosomal subunit RNA) rDNA (ribosomal DNA) sequences by a polymerase chain reaction-digoxigenin-labelling protocol, coupled to a capture oligonucleotide immobilized on a microtitre plate. The positive results are revealed by means of a colour reaction. In 6 h of non-intensive labour, we can detect as few as two to five clostridial cells or spores in experimentally contaminated soft drinks.