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Two-liquid-phase bioreactors

H M Van Sonsbeek1, H H Beeftink, J Tramper

  • 1Wageningen Agricultural University, Department of Food Science, The Netherlands.

Enzyme and Microbial Technology
|September 1, 1993
PubMed
Summary
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Two-liquid-phase systems enhance biocatalysis productivity and enable continuous processing. Overcoming challenges like interfacial effects and slow coalescence requires further research into bioreactor design and operation for successful scale-up.

Area of Science:

  • Biocatalysis and Chemical Engineering
  • Interface Science

Background:

  • Two-liquid-phase systems offer enhanced productivity and continuous processing for biocatalysis.
  • Challenges include interfacial effects (biocatalyst/medium component accumulation, activity loss) and slow coalescence.

Purpose of the Study:

  • To review recent advancements and proposals for two-liquid-phase bioreactors.
  • To discuss critical aspects for scale-up and further development of these systems.

Main Methods:

  • Review of existing literature on two-liquid-phase bioreactors.
  • Analysis of bioreactor configurations, including adaptations of loop and stirred-tank reactors.
  • Discussion of operational parameters such as aeration, shear stress, and processing modes (batch/continuous).

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Main Results:

  • Identified key limitations in two-liquid-phase biocatalysis, including interfacial phenomena and coalescence issues.
  • Highlighted the role of techniques like centrifugation, membranes, and immobilization in mitigating problems.
  • Presented various bioreactor configurations and adaptations for improved performance.

Conclusions:

  • Further fundamental understanding of interfaces and coalescence is crucial for successful application.
  • Strategic selection of bioreactor configuration and operational parameters is essential for process scale-up and development.