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Transformation of the acidophilic heterotroph Acidiphilium facilis by electroporation
K Inagaki1, J Tomono, N Kishimoto
1Division of Bioresources Science, Faculty of Agriculture, Okayama University, Japan.
Bioscience, Biotechnology, and Biochemistry
|October 1, 1993
Abstract:
We constructed a cloning vector for use in the acidophilic heterotroph Acidiphilium facilis. The vector pAH101 (8.8 kb) was constructed from a 6.1 kb restriction fragment of the Acidiphilium plasmid pAH1 and a pUC19 carrying a beta-lactamase gene. The antibiotic resistance gene was efficiently expressed in A. facilis. Several factors which influenced the transformation efficiency were optimized, resulting in a transformation efficiency of up to 3 x 10(3) transformants per microgram of plasmid DNA at a field strength of 10 kV/cm with a 7.0 ms pulse.