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Updated: Aug 11, 2026

09:21
Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor
Published on: January 4, 2016
Abstract:
1. The use of enzymes and thin-layer chromatography (TLC) in quantifying individual bile acids from bile or serum is reviewed. 2. Both 3alpha-hydroxysteroid dehydrogenase (commercially available P. testosteroni enzyme) and 7alpha-hydroxysteroid dehydrogenase (E. coli) can be used for spectrophotometric or fluorometric measurement of individual bile acids in TLC eluates and to estimate pool size and half-life of serially labelled samples.
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