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Related Experiment Videos

On-line immunoanalysis for bioprocess control

C Middendorf1, B Schulze, R Freitag

  • 1Abteilung Biotechnologie, Westfälische Wilhelms-Universität Münster, Germany.

Journal of Biotechnology
|December 1, 1993
PubMed
Summary
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New immunoassays combined with flow injection analysis (FIA) offer automated on-line monitoring for protein production. These turbidimetric immuno-FIA methods effectively analyze proteins like pullulanase and IgG during cultivation.

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Bioprocess Engineering

Background:

  • Enzyme-linked immunosorbent assays (ELISA) are common for protein detection in bioprocesses.
  • Traditional ELISA is time-consuming and difficult to automate, hindering real-time monitoring.
  • There is a need for efficient on-line methods to track protein production.

Purpose of the Study:

  • To develop and evaluate novel immuno-FIA methods for on-line protein analysis.
  • To demonstrate the effectiveness of turbidimetric immuno-FIA for monitoring cultivation processes.
  • To investigate the application of immuno-FIA for detecting various proteins.

Main Methods:

  • Developed heterogeneous and turbidimetric immuno-FIA techniques.
  • Applied FIA methods to detect thermostable pullulanase, IgG, antithrombin III, and recombinant tissue-type plasminogen activator.

Related Experiment Videos

  • Utilized turbidimetric immuno-FIA for on-line analysis of pullulanase and monoclonal mouse IgG in cultivation.

    • Main Results:

    • Successfully adapted immunoassays with FIA for protein detection.
    • Demonstrated the feasibility of on-line monitoring using turbidimetric immuno-FIA.
    • Validated the application of these methods for specific proteins like pullulanase and IgG.

    Conclusions:

    • Immuno-FIA methods provide an effective solution for automated on-line protein monitoring in bioprocesses.
    • Turbidimetric immuno-FIA is particularly suitable for real-time analysis of protein production.
    • These techniques enhance bioprocess control and efficiency through rapid analysis.