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Primary structure of the ADE1 gene from Candida utilis
1Tsuruga Institute of Biotechnology, Toyobo Co., Ltd., Fukui, Japan.
Bioscience, Biotechnology, and Biochemistry
|January 1, 1994
Summary
The ADE1 gene from Candida utilis was cloned and sequenced. Its amino acid sequence showed high homology to ADE1 genes in other yeast species, indicating conserved function in SAICAR synthetase.
Area of Science:
- * Molecular biology and yeast genetics.
- * Enzyme and gene characterization.
Background:
- * Candida utilis CA(u)-37 is commercially used for enzyme production.
- * The ADE1 gene is crucial for purine biosynthesis in yeast.
Purpose of the Study:
- * To clone and characterize the ADE1 gene from Candida utilis.
- * To investigate the homology and potential function of the Candida utilis ADE1 gene.
Main Methods:
- * Cloning of the ADE1 gene by complementation of an ade1 mutation in Saccharomyces cerevisiae.
- * DNA sequencing of the cloned gene.
- * Homology search and sequence analysis of the deduced amino acid sequence.
Main Results:
- * The ADE1 gene from Candida utilis CA(u)-37 was successfully cloned.
- * The gene consists of 903 base pairs.
- * The deduced amino acid sequence exhibited 70% homology to ADE1 genes from Saccharomyces cerevisiae and Candida maltosa, including a conserved SAICAR synthetase region.
Conclusions:
- * The cloned Candida utilis ADE1 gene encodes a functional SAICAR synthetase.
- * The high homology suggests conserved function and evolutionary relatedness among yeast ADE1 genes.