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Hydrolysis of S-2-aminoethylcysteinyl peptide bond by Achromobacter protease I
T Masaki1, T Takiya, S Tsunasawa
1Department of Resource Biology, Faculty of Agriculture, Ibaraki University, Japan.
Bioscience, Biotechnology, and Biochemistry
|January 1, 1994
Abstract:
The substrate specificity of Achromobacter protease I (API) was examined for S-2-aminoethyl(AE)cysteinyl bonds in Bz-AEC-OMe/OEt, Bz-AEC-NH2, and AE-insulin B chain. The protease hydrolyzed all of the tested AE-cysteinyl bonds at the same rate as that of lysyl bonds. Kinetic parameters were estimated for this hydrolysis reaction.