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Repeated hybridoma batch culture with cell recycle
1Glaxo Institute for Molecular Biology, Geneva, Switzerland.
Cytotechnology
|January 1, 1993
Summary
Recycling hybridoma cells after monoclonal antibody production boosts yields. This method doubles reactor productivity, allowing repeated antibody production cycles with fresh medium and similar antibody output per cycle.
Area of Science:
- Biotechnology
- Cell Culture
- Immunology
Background:
- Hybridoma cell cultures are typically discarded post-production.
- Monoclonal antibody (MAb) production is crucial in biotechnology.
- Optimizing MAb production efficiency is an ongoing challenge.
Purpose of the Study:
- To investigate the feasibility of recycling hybridoma cells for continuous MAb production.
- To assess the impact of cell recycling on antibody yield and reactor productivity.
- To evaluate the potential for repeated MAb production cycles.
Main Methods:
- Aseptic recycling of hybridoma cells into a stirred tank reactor.
- Addition of fresh medium to resume production after cell separation.
- Consecutive batch cycles with recycled cells were performed.
- Monoclonal antibody yield and volumetric productivity were measured.
Main Results:
- Antibody yields per cycle were comparable to standard batch cultures.
- 2.8 g of monoclonal antibody (MAb) was produced in 6 days using a 15 L reactor.
- Reactor volumetric productivity (mg/L/day) was doubled compared to standard batch operations.
Conclusions:
- Aseptic cell recycling is a viable strategy for enhancing hybridoma culture efficiency.
- This approach significantly increases MAb production rates.
- Repeated MAb production cycles are achievable with consistent yields.