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Immunochemical detection using the light-addressable potentiometric sensor

P R Panfili1, K Dill, J D Olson

  • 1Molecular Devices Corporation, Menlo Park.

Current Opinion in Biotechnology
|February 1, 1994
PubMed
Summary
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New assay systems using filtration capture and sensor detection provide rapid, sensitive results for immunoassays and binding studies. These methods achieve high sensitivity (10(-12) M) and complete assays in under 15 minutes.

Area of Science:

  • Biochemical assays
  • Analytical chemistry
  • Molecular biology

Background:

  • Immunoassays, receptor-ligand binding studies, and DNA probe assays require rapid, sensitive, and flexible analytical systems.
  • Existing methods may face limitations in speed, sensitivity, or flexibility for certain applications.

Purpose of the Study:

  • To present filtration capture and sensor detection as advantageous techniques for various bioanalytical assays.
  • To highlight the potential for high sensitivity and rapid assay times using these methods.

Main Methods:

  • Utilized filtration capture techniques for sample preparation and analyte isolation.
  • Employed sensor detection for quantifying binding events or target molecules.
  • Assessed assay performance based on sensitivity and assay duration.

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Main Results:

  • Demonstrated high sensitivity achievable with filtration capture and sensor detection, reaching the order of 10(-12) M.
  • Showcased the rapid nature of these assay systems, with total assay times under 15 minutes.
  • Highlighted the flexibility of the combined approach for diverse assay types.

Conclusions:

  • Filtration capture and sensor detection offer a powerful combination for developing advanced bioanalytical assays.
  • These techniques meet the demand for rapid, sensitive, and flexible assay systems in fields like immunoassays and molecular diagnostics.
  • The performance is dependent on the specific binding reagents used, but the platform offers significant advantages.