Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Drug synthesis by genetically engineered microorganisms

C R Hutchinson1

  • 1School of Pharmacy, University of Wisconsin, Madison 53706.

Bio/Technology (Nature Publishing Company)
|April 1, 1994
PubMed
Summary

Microbial drug discovery is advancing through genetic engineering. Genetically modified microorganisms can produce novel drug compounds and increase yields of existing antibiotics, expanding their role in pharmaceutical research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nonstereospecific hydrogen exchange in the biosynthesis of the macrolide antibiotic, brefeldin A.

Journal of the American Chemical Society·2011
Same author

Quantitative atom probe analysis of nanostructure containing clusters and precipitates with multiple length scales.

Ultramicroscopy·2011
Same author

Engineered biosynthesis of geldanamycin analogs for Hsp90 inhibition.

Chemistry & biology·2004
Same author

Approaches to stabilization of inter-domain recombination in polyketide synthase gene expression plasmids.

Journal of industrial microbiology & biotechnology·2003
Same author

A comparison of EDS microanalysis in FIB-prepared and electropolished TEM thin foils.

Ultramicroscopy·2002
Same author

Combinatorial biosynthesis in microorganisms as a route to new antimicrobial, antitumor and neuroregenerative drugs.

Current opinion in investigational drugs (London, England : 2000)·2002

Area of Science:

  • Microbiology
  • Biotechnology
  • Medicinal Chemistry

Background:

  • The integration of chemical and biological methods in drug discovery is accelerating.
  • Combinatorial methods enhance screening program output.
  • Genetically modified microorganisms offer new avenues for metabolite production.

Purpose of the Study:

  • To explore the potential of genetically modified microorganisms in drug discovery.
  • To highlight the role of manipulating microbial biosynthetic genes for novel drug analogs.
  • To emphasize the synergy between genetic engineering and traditional drug research.

Main Methods:

  • Manipulation of Type II polyketide synthase genes in Actinomycetes.
  • Targeted mutation of specific biosynthetic genes for macrolide antibiotic analogs.
  • Genetic alteration of pathways for aminoglycoside and oligopeptide antibiotics.
  • DNA-based prescreening of microbial isolates for pharmacologically active genes.

Main Results:

  • Actinomycetes can produce novel aromatic compounds and unique macrolide antibiotic analogs.
  • Genetic engineering enables the production of analogs not achievable through chemical synthesis.
  • Altering biosynthetic pathways offers promising approaches for novel metabolite manufacturing.
  • DNA-based prescreening identifies microbial isolates with potential drug-related genes.

Conclusions:

  • Genetically modified microorganisms are increasingly vital in drug research and development.
  • Microbial manipulation provides access to novel chemical entities and improved antibiotic production.
  • The combination of genetic approaches and prescreening expands the role of microorganisms in discovering new drugs.

Related Experiment Videos