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Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

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Published on: December 15, 2017

Suboptimal control of fed-batch bioprocesses using phase properties

A Diener¹, B Goldschmidt

¹Martin-Luther-University Halle, Institute of Biotechnology, Germany.

Journal of Biotechnology

|March 15, 1994

Summary

A novel method optimizes fed-batch bioprocess feeding strategies using time-local optimization. This robust approach enhances process control and can be applied online, even with complex models and constraints.

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Light-Controlled Fermentations for Microbial Chemical and Protein Production

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Area of Science:

Bioprocess Engineering
Chemical Engineering
Control Theory

Background:

Fed-batch bioprocesses are crucial for producing valuable compounds.
Optimizing feeding strategies is essential for maximizing yield and efficiency.
Existing methods may struggle with complex dynamics and constraints.

Purpose of the Study:

Introduce a new method for evaluating suboptimal feeding strategies in fed-batch bioprocesses.
Develop a robust and adaptable optimization technique for bioprocess control.
Demonstrate the method's applicability using a penicillin bioprocess.

Main Methods:

Time-local optimization of process dynamics.
Partitioning the process into phases with distinct local extremality conditions.
Developing a feedback control law derived from the optimization.

Main Results:

The proposed method effectively evaluates feeding strategies by incorporating global effects through phase partitioning.
The penicillin bioprocess example illustrates the method's practical application.
The evaluated control function is presented as a feedback law.

Conclusions:

The new method offers a robust and stable numerical procedure suitable for on-line bioprocess controls.
It allows for the inclusion of process state constraints and complex models.
The feedback-based control enhances adaptability and resilience to perturbations.