Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Metabolic engineering

G Stephanopoulos1

  • 1Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge 02139.

Current Opinion in Biotechnology
|February 1, 1994
PubMed
Summary
This summary is machine-generated.

Metabolic engineering purposefully designs entire metabolic networks, not just individual reactions. This approach aims to create novel pathways for superior strain yield and productivity using advanced analysis methods.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Improving CRISPR/Cas9-mediated genome editing efficiency in Yarrowia lipolytica using direct tRNA-sgRNA fusions.

Metabolic engineering·2020
Same author

Eliciting the impacts of cellular noise on metabolic trade-offs by quantitative mass imaging.

Nature communications·2019
Same author

Engineering Microbes to Synthesize Plant Isoprenoids.

Methods in enzymology·2016
Same author

Origins of Cell-to-Cell Bioprocessing Diversity and Implications of the Extracellular Environment Revealed at the Single-Cell Level.

Scientific reports·2015
Same author

Metabolic pathway alterations that support cell proliferation.

Cold Spring Harbor symposia on quantitative biology·2012
Same author

A novel bioreactor-cell precipitator combination for high-cell density, high-flow fermentations.

Biotechnology progress·2010
Same journal

Microbial C1 assimilation pathways for chemical synthesis: from native metabolism to synthetic design.

Current opinion in biotechnology·2026
Same journal

Medicinal plants fermentation: current knowledge and perspectives.

Current opinion in biotechnology·2026
Same journal

Fermented foods: lessons learned from metagenomics.

Current opinion in biotechnology·2026
Same journal

Microfluidic platforms for the transient transfection of mammalian cells: recent developments and challenges.

Current opinion in biotechnology·2026
Same journal

Harvesting insights from recent advances in yeast genomics for predictable and precision wine fermentation.

Current opinion in biotechnology·2026
Same journal

Minimal enzyme cascades for the aromatic-to-aromatic upgrading of lignin monomers.

Current opinion in biotechnology·2026
See all related articles

Area of Science:

  • Biotechnology
  • Systems Biology

Background:

  • Metabolic engineering involves purposeful design of metabolic networks.
  • It differs from classic manipulation of intermediary metabolism.

Purpose of the Study:

  • To construct novel pathways and thermodynamically feasible reaction networks.
  • To identify limiting branch-points and enzymatic reactions for strain improvement.

Main Methods:

  • Examination of biochemical reactions in their entirety.
  • Application of Metabolic Control Analysis (MCA).
  • Experimental determination of elasticities and flux control coefficients.

Main Results:

  • Development of methodologies for experimental determination of elasticities and flux control coefficients.

Related Experiment Videos

  • Progress toward constructing strains with superior yield and productivity.

    • Conclusions:

    • Metabolic engineering offers a systematic approach to designing metabolic networks.
    • MCA provides tools for understanding and optimizing metabolic pathways for enhanced biological production.