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Updated: Jul 29, 2026

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
Published on: May 1, 2012
Problems and approaches in covalent attachment of peptides and proteins to inorganic surfaces for biosensor
J E Zull1, J Reed-Mundell, Y W Lee
1Department of Biology, Case Western Reserve University, Cleveland, OH 44106.
Abstract:
Some of the fundamental problems in covalent attachment of peptides and proteins to putative biosensor surfaces are reviewed and specific approaches to these problems discussed. In addition, selected aspects of our recent work utilizing self-assembled monolayer (SAM) systems designed to react selectively with the thiol side chain of Cys in proteins are presented. Uniform attachment of a 21-amino acid peptide antigen through a single Cys residue with retention of biological function (antibody binding) has been attained. Further work with this system may lead to solutions for some of the problems which currently prevent the development of reliable biosensors for industrial and medical use.
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