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Preparation and characterization of anti-fumonisin monoclonal antibodies
S Fukuda1, A Nagahara, M Kikuchi
1Research and Development Division, Kikkoman Corporation, Chiba, Japan.
Bioscience, Biotechnology, and Biochemistry
|April 1, 1994
Abstract:
Murine monoclonal antibodies were prepared against fumonisin B1-carrier protein conjugates, such as fumonisin B1-ovalbumin and fumonisin B1-keyhole limpet hemocyanin. Five stable hybridoma clones were obtained. The response ranges of these monoclonal antibodies for fumonisin B1 were from 10 to 1000-5000 ng/ml in the competitive indirect enzyme-linked immunosorbent assay (CI-ELISA).