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Current research to develop the entomopathogen Bacillus thuringiensis for horn fly control
1Knipling-Bushland U.S. Livestock Insects Research Laboratory, U.S. Department of Agriculture, Kerrville, TX 78028-9184.
SAAS Bulletin, Biochemistry and Biotechnology
|January 1, 1994
Summary
Bacillus thuringiensis israelensis (Bti) proteins show larvicidal activity against horn flies. Recombinant DNA technology offers a feasible path for developing new Bti-based pest control strategies.
Area of Science:
- Microbiology
- Entomology
- Biotechnology
Background:
- Bacillus thuringiensis subspecies israelensis (Bti) produces proteins with larvicidal properties.
- Horn flies (Haematobia irritans L.) are significant agricultural pests.
Purpose of the Study:
- To investigate the potential of Bti for controlling horn flies.
- To explore the feasibility of using recombinant DNA technology for developing Bti-based pest control.
Main Methods:
- Bioassays were conducted using Bti proteins against horn fly larvae.
- Recombinant DNA technology was considered for enhancing Bti's efficacy.
Main Results:
- Bti demonstrated larvicidal activity against horn flies in bioassays.
- The study identified at least two sporulation-specific proteins responsible for this activity.
Conclusions:
- Bti shows promise as a biological control agent for horn flies.
- Further research and development of molecular tools are necessary to realize Bti-based pest control solutions.