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DNA fingerprinting using arbitrary primer technology (APT): a tool or a torment
1Cooperative Research Centre for Tropical Plant Pathology, University of Queensland, St Lucia, Australia.
Australasian Biotechnology
|July 1, 1994
Summary
Arbitrary primer technology (APT) offers an easy way to create DNA fingerprints without prior sequence knowledge. This discussion addresses concerns about reproducibility and resolution in APT methods like RAPD, DAF, and AP-PCR.
Area of Science:
- Molecular Biology
- Genetics
Background:
- Polymerase Chain Reaction (PCR) variations enable DNA fingerprinting independent of prior sequence data.
- Arbitrary Primer Technology (APT) encompasses methods like Random Amplified Polymorphic DNA (RAPD), DNA Amplification Fingerprinting (DAF), and Arbitrary Primer PCR (AP-PCR).
Purpose of the Study:
- To discuss the reproducibility and resolution of APT methods.
- To examine the unique properties of DNA amplification using short arbitrary primers.
Main Methods:
- Review and discussion of arbitrary primer technology (APT) principles.
- Analysis of DNA amplification using single short arbitrary primers.
Main Results:
- APT methods provide ease and independence from sequence information for DNA fingerprinting.
- Questions regarding reproducibility and resolution of APT techniques have been raised.
Conclusions:
- Arbitrary primer technology presents a powerful tool for DNA fingerprinting.
- Further examination of APT properties is warranted to address concerns and optimize its application.