Jove
Visualize
Contact Us

Related Experiment Videos

P-glycoprotein structure and evolutionary homologies

J M Croop1

  • 1Division of Pediatric Oncology, Dana-Farber Cancer Institute, Boston, MA.

Cytotechnology
|January 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

P-glycoprotein structure and evolutionary homologies.

Cytotechnology·2008
Same author

Mobilization and collection of peripheral blood CD34+ cells from patients with Fanconi anemia.

Blood·2001
Same author

Large-scale mobilization and isolation of CD34+ cells from normal donors.

Bone marrow transplantation·2001
Same author

Evolutionary relationships among ABC transporters.

Methods in enzymology·1998
Same author

Multidrug resistance gene expression correlates with progesterone production in dehydroepiandrosterone-induced polycystic and equine chorionic gonadotropin-stimulated ovaries of prepubertal rats.

Biology of reproduction·1998
Same author

Gene immunotherapy in murine acute myeloid leukemia: granulocyte-macrophage colony-stimulating factor tumor cell vaccines elicit more potent antitumor immunity compared with B7 family and other cytokine vaccines.

Blood·1998
Same journal

Combination of paclitaxel with eugenol effectively enhances anticancer characteristics of paclitaxel on the A549 lung cancer cell line.

Cytotechnology·2026
Same journal

RBM15 enhances ILDR1 n6-methyladenosine methylation to accelerate gastric adenocarcinoma progression via an IGF2BP3-dependent manner.

Cytotechnology·2026
Same journal

Apigenin attenuates hepatocyte senescence in metabolic dysfunction-associated steatotic liver disease by targeting the TGF-β signaling pathway.

Cytotechnology·2026
Same journal

Iodine-125 seed induces ferroptosis through inhibiting RNF216-mediated ubiquitination of p53 in cholangiocarcinoma cells.

Cytotechnology·2026
Same journal

Magnetic resonance imaging-based Alzheimer's disease detection using an EfficientNet-CMSACCN framework.

Cytotechnology·2026
Same journal

Brusatol Induces Ferroptosis in Condyloma Acuminatum Keratinocytes by Suppressing Nrf2 via Inhibition of the PI3K/AKT Pathway.

Cytotechnology·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Multidrug resistance in cells is linked to P-glycoprotein, a membrane protein family. These proteins transport diverse agents using ATP, and their evolutionary origins are explored through sequence analysis.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Multidrug resistance (MDR) is a significant challenge in medicine, often mediated by efflux pumps.
  • P-glycoproteins (PGPs) are key membrane proteins involved in MDR, identified through analysis of resistant cell lines.

Purpose of the Study:

  • To investigate the P-glycoprotein multigene family and its role in cellular resistance.
  • To understand the evolutionary origins of P-glycoproteins by comparing their structure and sequences across species.

Main Methods:

  • Analysis of multidrug resistant cell lines.
  • Characterization of P-glycoprotein homologs from various species.
  • Comparison of amino acid sequences, structural similarities, and intron/exon boundaries.

Related Experiment Videos

Main Results:

  • Identification of the P-glycoprotein multigene family, with specific classes conferring broad-spectrum resistance to cytotoxic agents.
  • Detailed structural analysis revealing P-glycoproteins as integral membrane glycoproteins with conserved domains.
  • Comparative analysis of homologs suggests evolutionary relationships within the P-glycoprotein superfamily.

Conclusions:

  • P-glycoproteins are crucial ATP-dependent transporters involved in cellular defense mechanisms.
  • Understanding P-glycoprotein structure and evolution provides insights into drug resistance and transporter function.
  • Further research into P-glycoprotein homologs can reveal novel therapeutic targets.