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A model system for the continuous production of a heterologous protein using a novel secretion promoting factor which
K Tokugawa1, T Ishii, K Nakamura
1Central Laboratories for Key Technology, KIRIN Brewery Co., Ltd., Yokohama, Japan.
Journal of Biotechnology
|September 15, 1994
Abstract:
The 'PAS factor' whose gene has been cloned from a species of Vibrio, is a novel protein secretion factor which is functional in Escherichia coli cells. To demonstrate that practical use of the PAS factor gene is possible, we have constructed a model secretion vector 'pAS23'. Using this system, beta-lactamase was produced and secreted into the medium of a continuous culture system, after optimization of culture conditions.