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Determination of acid phosphatase in cultivation medium using asymmetrical flow field-flow fractionation
A Litzén1, M B Garn, H M Widmer
1Corporate Analytical Research, Ciba-Geigy, Ltd., Basel, Switzerland.
Journal of Biotechnology
|November 15, 1994
Abstract:
Highly glycosylated proteins and proteins larger than 100,000 Da are difficult to analyze in complex media with standard liquid chromatography techniques. Asymmetrical flow field-flow fractionation is a simple bio-compatible method for such analysis which can also be used for on-line monitoring. A method for determination of acid phosphatase (EC 3.1.3.2), molecular weight 1,000,000, produced by a recombinant DNA yeast strain is presented.