Ammonium-mediated reduction of plasmid copy number and recombinant gene expression in Escherichia coli

The effect of ammonium as a medium supplement on plasmid-encoded recombinant beta-galactosidase synthesis was explored in Escherichia coli cells during aerobic growth in complex medium. After induction, only doses of ammonium chloride below 1 g/L are able to transiently enhance the yield. However, the presence of nontoxic ammonium chloride concentrations of up to 10 g/L results in lower values of beta-galactosidase in a concentration-dependent fashion. A significant reduction in plasmid DNA content explains the decrease in the yield by a gene-dosage-involving mechanism.