Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Transformation in Aspergillus ochraceus

D Saha1, T K Das

  • 1Department of Biochemistry and Biophysics, University of Kalyani, West Bengal, India.

Current Microbiology
|February 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Clinical Characteristics and Laboratory Findings of Early and Late Onset Pediatric Idiopathic Intracranial Hypertension: A Single-Centre Experience.

Mymensingh medical journal : MMJ·2026
Same author

The Effect of Combined Iron and Methylphenidate Use in Iron Deficient Attention deficit Hyperactivity Disorder (ADHD) Children of Bangladesh.

Mymensingh medical journal : MMJ·2026
Same author

Understanding phosphorus dynamics and uptake in diverse soils of Indo-Gangetic Plains of India.

PloS one·2025
Same author

Does childhood TB or pneumonia impact COPD or asthma in adulthood?

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2025
Same author

The interplay between external residue addition, and soil organic carbon dynamics and mineralization kinetics: Experiences from a 12-year old conservation agriculture.

Journal of environmental management·2024
Same author

Modeling maize growth and nitrogen dynamics using CERES-Maize (DSSAT) under diverse nitrogen management options in a conservation agriculture-based maize-wheat system.

Scientific reports·2024
Same journal

Construction of an Oral Vaccine Based on Multiple Antigens of African Swine Fever Virus Displayed on the Surface of Bacillus Subtilis Spores and Study of its Immune Effect in Rats.

Current microbiology·2026
Same journal

Next Generation Probiotics Challenges: A Road to use as Live Bio-Therapeutics.

Current microbiology·2026
Same journal

The Sea Cucumber Holobiont and Probiotics: Recent Progress on Apostichopus japonicus.

Current microbiology·2026
Same journal

Promoter Engineering of the Surfactin Operon Enhances Surfactin Production in the Environmental Strain Bacillus subtilis RI4914.

Current microbiology·2026
Same journal

Staphylococcus aureus CC398 Lineage of the Human Clade Isolated from Bloodstream Infection and Colonization and Spread among Brazilian Patients Hospitalized during the COVID-19 Pandemic.

Current microbiology·2026
Same journal

Evaluation of the Immunogenicity of an Inactivated Vaccine against the Adenovirus Egg Drop Syndrome 1976 in Poultry.

Current microbiology·2026
See all related articles

This study investigated DNA transformation in Aspergillus ochraceus mutants. Donor DNA transforming activity was affected by heat, pH, starvation duration, and DNA concentration.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Aspergillus ochraceus is a filamentous fungus with industrial applications.
  • Understanding DNA transformation is crucial for genetic manipulation in fungi.

Purpose of the Study:

  • To investigate the transformation efficiency of Aspergillus ochraceus using donor DNA.
  • To identify factors affecting the transforming activity of donor DNA.

Main Methods:

  • Lysine-requiring mutants of Aspergillus ochraceus were subjected to starvation.
  • Mutants were treated with DNA from a wild-type strain.
  • Transformation efficiency was assessed by colony counting after growth on minimal medium.

Main Results:

Related Experiment Videos

  • The transforming activity of donor DNA was sensitive to heat and pH variations.
  • Transformation efficiency was influenced by the duration of starvation.
  • Donor DNA concentration significantly impacted the percentage of transformants.

    • Conclusions:

    • Factors such as heat, pH, starvation period, and donor DNA concentration modulate DNA transformation in Aspergillus ochraceus.
    • These findings provide insights into optimizing genetic transformation protocols for this fungus.