Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Extracellular insulin degrading activity creates instability in a CHO-based batch-refeed continuous process

D Drapeau1, Y T Luan, J A Popoloski

  • 1Genetics Institute, Inc., Andover MA 01810.

Cytotechnology
|January 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Comparative analysis of intestinal microbiota distribution characteristics based on metagenomics in patients with hepatitis B cirrhosis with or without ascites].

Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology·2023
Same author

The Port Health Service.

The Medical journal of the South-West·2017
Same author

Stricture formation following sulphonamide therapy of gonorrhoea.

The British journal of venereal diseases·2010
Same author

Validation of administrative data used for the diagnosis of upper gastrointestinal events following nonsteroidal anti-inflammatory drug prescription.

Alimentary pharmacology & therapeutics·2006
Same author

Fecundity and egg output by Toxocara canis in the red fox, Vulpes vulpes.

Journal of helminthology·2001
Same author

Viral safety of B-domain deleted recombinant factor VIII.

Seminars in hematology·2001

Cellular insulin degradation activity caused process upsets in recombinant Chinese hamster ovary cell cultures. This degradation, released during preceding passages, led to early insulin depletion, halting cell growth and reducing viability.

Area of Science:

  • Biotechnology
  • Cell Culture Technology
  • Bioprocess Engineering

Background:

  • Recombinant Chinese hamster ovary (CHO) cell lines are crucial for biopharmaceutical production.
  • Batch-refeed continuous processes are widely used for large-scale cell culture.
  • Intermittent process upsets can negatively impact productivity and product quality.

Purpose of the Study:

  • To investigate the cause of occasional process upsets in a batch-refeed continuous culture of CHO cells.
  • To identify the factors contributing to halted cell growth and reduced viability.
  • To understand the role of insulin in the observed cell culture instability.

Main Methods:

  • Monitoring cell growth, viability, and key media components during batch-refeed continuous culture.

Related Experiment Videos

  • Analyzing insulin levels and identifying potential degradation factors in spent medium.
  • Correlating process upsets with specific events or conditions in preceding culture passages.

    • Main Results:

    • Process upsets were consistently associated with early depletion of insulin during a passage.
    • Insulin depletion was primarily caused by an insulin-degrading activity released by CHO cells.
    • This degrading activity was found to be present in the medium from the preceding cell culture passage.

    Conclusions:

    • Cellular release of insulin-degrading activity is a key factor causing instability in CHO cell batch-refeed continuous processes.
    • Proactive management of insulin levels and mitigation of cellular degradation activity are essential for robust bioprocesses.
    • Understanding and controlling intracellular and extracellular factors affecting nutrient stability is critical for optimizing recombinant protein production.