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Low temperature cultivation--a step towards process optimisation

R Weidemann1, A Ludwig, G Kretzmer

  • 1Institut für Technische Chemie, Hannover FRG.

Cytotechnology
|January 1, 1994
PubMed
Summary
This summary is machine-generated.

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Cultivating recombinant BHK cells at lower temperatures (30-37°C) reduced growth and glucose consumption but maintained cell density and productivity. This indicates temperature optimization for cell culture metabolism.

Area of Science:

  • Biotechnology
  • Cell Biology
  • Bioprocess Engineering

Background:

  • Mammalian cell culture is crucial for biopharmaceutical production.
  • Optimizing culture conditions, such as temperature, can significantly impact cell metabolism and productivity.
  • Recombinant Baby Hamster Kidney (BHK) cells are widely used in bioprocessing.

Purpose of the Study:

  • To investigate the effect of cultivation temperature on the metabolism and growth of adherent recombinant BHK cells.
  • To determine optimal temperature ranges for BHK cell cultivation in bioreactors.

Main Methods:

  • Adherent recombinant BHK cells were cultured in a 2-litre bioreactor.
  • Batch and repeated-batch cultivation strategies were employed.
  • Cultivations were performed at temperatures ranging from 30 to 37 degrees C.

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Main Results:

  • Lower cultivation temperatures (closer to 30°C) resulted in reduced specific growth rates.
  • Glucose consumption rates decreased at lower temperatures, leading to diminished lactate production.
  • Maximum cell densities and overall productivity were not significantly affected by temperature reduction within the tested range.

Conclusions:

  • Temperature significantly influences BHK cell metabolism, specifically growth rate and substrate consumption.
  • Lower temperatures can be beneficial for reducing metabolic byproducts like lactate without compromising cell density or productivity.
  • Findings suggest potential for optimizing bioreactor temperature for enhanced bioprocess efficiency and product quality.