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A direct computer control concept for mammalian cell fermentation processes

H Büntemeyer1, R Marzahl, J Lehmann

  • 1Institute of Cell Culture Technology, University of Bielefeld, Germany.

Cytotechnology
|January 1, 1994
PubMed
Summary
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A new personal computer-based system offers advanced control for laboratory-scale bioreactors. This system automates complex animal cell culture processes, including aeration and data acquisition, for enhanced fermentation management.

Area of Science:

  • Biotechnology
  • Bioprocess Engineering
  • Cell Culture Technology

Background:

  • Mammalian cell culture demands sophisticated fermentation setups.
  • Continuous fermentation and cell retention require precise process control.
  • University laboratories face unique challenges in animal cell culture.

Purpose of the Study:

  • To present a novel control and data-acquisition system for laboratory-scale bioreactors.
  • To develop user-oriented software for complex animal cell culture requirements.
  • To automate standard operations and manage diverse fermentation strategies.

Main Methods:

  • Utilized a standard personal computer connected to sensors, pumps, and valves via Analog-to-Digital (ADA) transformation.
  • Developed freely programmable, user-oriented software for integrated control.

Related Experiment Videos

  • Implemented direct digital control (ddc) for oxygen partial pressure and pH regulation.

    • Main Results:

    • Achieved integrated control of aeration, pumps, data acquisition, and storage within a single program.
    • Enabled automation of key measurements like oxygen transfer rate (OTR) and oxygen mass transfer coefficient (kLa).
    • Included pump control algorithms for batch, fed-batch, chemostat, and perfusion fermentation strategies.

    Conclusions:

    • The presented system effectively manages complex laboratory-scale bioreactors for animal cell culture.
    • The software facilitates automation and flexible control over various fermentation modes.
    • Direct digital control ensures straightforward adaptation of parameters for optimal fermentation conditions.