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Related Experiment Videos

Cells and bubbles in sparged bioreactors

J J Chalmers1

  • 1Department of Chemical Engineering, Ohio State University, Columbus 43210.

Cytotechnology
|January 1, 1994
PubMed
Summary

Gas bubbles, commonly used for oxygen in cell culture, can harm animal cells. This review summarizes research on bubble-induced cell damage and identifies areas needing further investigation.

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Bioprocessing

Background:

  • In vitro cell culture commonly uses gas bubbles for oxygenation.
  • Empirical evidence suggests these bubbles can be detrimental to cell viability.

Purpose of the Study:

  • To review existing research on bubble-induced cell damage in vitro.
  • To synthesize current knowledge and identify knowledge gaps regarding bubble effects on cells.

Main Methods:

  • Literature review encompassing experimental data, hydrodynamics, bioreactor studies, and simulations.
  • Analysis of cell attachment to bubbles and bubble rupture mechanics.

Main Results:

  • Bubbles can cause significant cell damage through various mechanisms.
  • Bubble rupture is a key factor contributing to cell death in agitated cultures.

Conclusions:

  • Understanding bubble dynamics is crucial for optimizing in vitro cell culture conditions.
  • Further research is needed to fully elucidate bubble-induced cell death pathways and mitigation strategies.

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