Related Experiment Video
Updated: Sep 14, 2026

Isolation and Quantification of Botulinum Neurotoxin From Complex Matrices Using the BoTest Matrix Assays
Published on: March 4, 2014
Growth and toxin production by non-proteolytic and proteolytic Clostridium botulinum in cooked vegetables
Abstract:
Growth and toxin production by proteolytic and non-proteolytic strains of Clostridium botulinum have been followed in 28 cooked puréed vegetables prepared under strict anaerobic conditions and incubated at 30 degrees C for up to 60 d. Toxin production was confirmed in 25 of the cooked vegetables inoculated with a suspension of spores of proteolytic strains of types A and B, and in 13 inoculated with a suspension of spores of non-proteolytic strains of types B, E and F. For both proteolytic and non-proteolytic strains, a trend was identified correlating growth and toxin production with the pH of the cooked puréed vegetables.
Related Concept Videos
Microbes in the Production of Fermented Foods
Microbial Spoilage of Food
Sources of Food Contamination
Production of Biopesticides
Bacterial Toxins
Botulism

