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Nuclear disintegration in chicken peritoneal macrophages exposed to fumonisin B1 from Indian maize

D Chatterjee1, S K Mukherjee, A Dey

  • 1Department of Food Technology and Biochemical Engineering, Jadavpur University, Calcutta, India.

Letters in Applied Microbiology
|March 1, 1995
PubMed

Insights

Fumonisin B1 (FB1) exposure damages chicken macrophages, causing nuclear disintegration. Higher FB1 doses led to more severe damage, suggesting dietary FB1 may increase infection susceptibility in consumers.

Area of Science:

  • Veterinary immunology
  • Mycotoxicology
  • Cell biology

Background:

  • Macrophages are crucial immune cells in poultry.
  • Fumonisin B1 (FB1) is a mycotoxin found in contaminated feed.
  • Understanding FB1's impact on avian immunity is vital for animal health.

Purpose of the Study:

  • To investigate the morphological effects of FB1 on chicken peritoneal macrophages.
  • To determine the dose-dependent toxicity of FB1 on these immune cells.

Main Methods:

  • Chicken peritoneal macrophages were isolated.
  • Cells were exposed to varying concentrations of FB1 (6–18 µg/mL).
  • Morphological changes, specifically nuclear integrity, were assessed.

Main Results:

  • FB1 exposure induced significant nuclear disintegrations in macrophages.
  • A dose-dependent relationship was observed, with increasing FB1 concentrations correlating with higher rates of nuclear damage.
  • The threshold for observable damage was within the tested dose range.

Conclusions:

  • Dietary exposure to FB1 can compromise the integrity of chicken macrophages.
  • This cellular damage may impair immune function, increasing susceptibility to infections in poultry.
  • FB1 contamination in feed poses a potential risk to avian health.

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