Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural requirements for mitomycin C DNA bonding

V S Li1, D Choi, M S Tang

  • 1Department of Chemistry, University of Houston, Texas 77204-5641, USA.

Biochemistry
|May 30, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sol-gel synthesis and characterization of ZnO-SiO<sub>2</sub> nanocomposites: a comparative study with pure ZnO and SiO<sub>2</sub>.

Nanoscale advances·2025
Same author

Acute myocardial infarction occurring at pre-existing mild stenosis, on the image obtained 3 days before the onset of acute myocardial infarction.

BMJ case reports·2025
Same author

Spinal claudication due to myxopapillary ependymoma.

BMJ case reports·2025
Same author

Nocturnal Autonomic Nervous System Dynamics and Chronic Painful Temporomandibular Disorders.

JDR clinical and translational research·2025
Same author

Comparative Efficacy of Subcutaneous and Intravenous Infliximab and Vedolizumab for Maintenance Treatment of TNF-naive Adult Patients with Inflammatory Bowel Disease: A Systematic Literature Review and Network Meta-analysis.

Digestive diseases and sciences·2024
Same author

CT findings of inferior vena cava trauma according to the level of injury: a retrospective analysis of 19 cases in a single trauma centre.

Clinical radiology·2023
Same journal

Aromatic Cage-Directed Azide-Methyllysine Photochemistry for Profiling Nonhistone Interacting Partners of the MeCP2 Methyl-CpG-Binding Domain.

Biochemistry·2026
Same journal

Differential Hydroxypyruvate Processing by <i>E. coli</i> and <i>P. aeruginosa</i> DXP Synthases Reveals Preferential Xylulose 5-Phosphate Formation by the <i>P. aeruginosa</i> Enzyme.

Biochemistry·2026
Same journal

Structural and Functional Characterization of Heterologous Nitrogenase Complexes.

Biochemistry·2026
Same journal

Discovery of Bacterial Unspecific Peroxygenases.

Biochemistry·2026
Same journal

Lactate Biology: Subcellular Routing and Chemical Form Define Function.

Biochemistry·2026
Same journal

Nature's Anaerobic Toolkit: Glycyl Radical Enzymes and Their Expanding Functional and Mechanistic Diversity.

Biochemistry·2026
See all related articles

Researchers explored how modified mitomycins bind to DNA. Structural variations at C-9 and C-9a did not alter DNA bonding sites, suggesting aromatization precedes mitomycin C binding.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Medicinal Chemistry

Background:

  • Mitomycins are bioreductive alkylating agents with anticancer properties.
  • Understanding the mechanism of DNA binding is crucial for developing targeted therapies.

Purpose of the Study:

  • To elucidate the structural requirements for selective DNA bonding of activated mitomycin species.
  • To investigate the influence of substituents at the C-9 and C-9a positions on mitomycin's DNA binding profile.

Main Methods:

  • Synthesis of modified mitomycins with varied C-9 and C-9a substituents.
  • Determination of DNA bonding sequence selectivities under reductive conditions.
  • Comparative analysis of DNA binding profiles for mitomycin C, mitomycin-9a-sulfonate, and mitomycin D.

Related Experiment Videos

Main Results:

  • Mitomycin-9a-sulfonate and mitomycin D exhibited DNA bonding profiles similar to mitomycin C.
  • Neither the stereochemistry nor the leaving group identity at C-9a affected the DNA bonding sites.
  • Aromatization of the dihydropyrrole ring was identified as a prerequisite for mitomycin C DNA binding.

Conclusions:

  • The specific structure of activated mitomycin species for DNA bonding is primarily determined by the aromatization step.
  • Modifications at C-9 and C-9a positions do not dictate the sequence selectivity of DNA binding.
  • These findings provide insights into the structure-activity relationship of mitomycins for improved anticancer drug design.