Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The poly(dT).2poly(dA) triple helix

F B Howard1, H T Miles, P D Ross

  • 1Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diaseases, National Institutes of Health, Bethesda, Maryland 20892-0540, USA.

Biochemistry
|May 30, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A case series and literature review on patients with rhinological complications secondary to the use of cocaine and levamisole.

The Journal of laryngology and otology·2020
Same author

The impact of Scottish Government protocols on practice in Scotland.

The Journal of laryngology and otology·2014
Same author

Associations of vertebral deformities and osteoarthritis with back pain among Japanese women: the Hizen-Oshima study.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2012
Same author

Thermodynamics of macromolecular association reactions: analysis of forces contributing to stabilization.

Biophysical journal·2009
Same author

Vitamin D status and falls, frailty, and fractures among postmenopausal Japanese women living in Hawaii.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2009
Same author

Can we improve dysphagia referrals?

The Journal of laryngology and otology·2006
Same journal

Aromatic Cage-Directed Azide-Methyllysine Photochemistry for Profiling Nonhistone Interacting Partners of the MeCP2 Methyl-CpG-Binding Domain.

Biochemistry·2026
Same journal

Differential Hydroxypyruvate Processing by <i>E. coli</i> and <i>P. aeruginosa</i> DXP Synthases Reveals Preferential Xylulose 5-Phosphate Formation by the <i>P. aeruginosa</i> Enzyme.

Biochemistry·2026
Same journal

Structural and Functional Characterization of Heterologous Nitrogenase Complexes.

Biochemistry·2026
Same journal

Discovery of Bacterial Unspecific Peroxygenases.

Biochemistry·2026
Same journal

Lactate Biology: Subcellular Routing and Chemical Form Define Function.

Biochemistry·2026
Same journal

Nature's Anaerobic Toolkit: Glycyl Radical Enzymes and Their Expanding Functional and Mechanistic Diversity.

Biochemistry·2026
See all related articles

A new triple helix, (dT)n.2(dA)n, forms from DNA duplexes at high salt concentrations. Its stability is influenced by temperature, salt type, and concentration, with implications for DNA structure.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • DNA can form complex structures beyond the canonical double helix.
  • Polynucleotide triple helices are of interest for understanding DNA structure and function.
  • The formation and stability of these structures are influenced by environmental factors like salt concentration.

Purpose of the Study:

  • To investigate the formation and characteristics of a novel homopolynucleotide triple helix, (dT)n.2(dA)n.
  • To determine the thermodynamic parameters governing the formation of this triple helix.
  • To explore the influence of salt concentration and type on triple helix stability.

Main Methods:

  • Circular dichroism spectroscopy was used to detect and characterize the triple helix formation.

Related Experiment Videos

  • Differential scanning calorimetry was employed to study the thermodynamic properties.
  • UV-Vis spectroscopy and melting temperature (Tm) analyses were conducted under varying salt conditions.

    • Main Results:

    • A new homopolynucleotide triple helix, (dT)n.2(dA)n, was identified, forming via an endothermic reaction.
    • Triple helix formation is favored by high salt concentrations ([NaCl] ≥ 2.6 M) and moderate temperatures.
    • Stability is entropy-driven, with unfavorable enthalpy changes; stability decreases with increasing salt concentration and is affected by specific anions (Cl-, Br-, ClO4-).

    Conclusions:

    • The (dT)n.2(dA)n triple helix is a weakly stabilized complex with thermodynamic properties sensitive to ionic strength.
    • The findings provide insights into the interactions between polynucleotides and ions, relevant to DNA structural transitions.
    • The study proposes a bonding scheme and discusses implications for related triple helical structures, such as Py.Pu.Pu helices.