Changes in the molecular types of connectin and nebulin during development of chicken skeletal muscle

Changes in the molecular types of connectin and nebulin during development of chicken breast and leg muscles were determined by an improved SDS-polyacrylamide gel electrophoresis (PAGE) using 2% polyacrylamide slab gel. The adult leg-type alpha-connectin (alpha L-connectin) and nebulin (L-nebulin) appeared in embryonic breast muscle, and changed into the adult breast-type ones (alpha B-connectin, B-nebulin) specific for adult breast muscle after hatching. In leg muscle, alpha L-connectin and L-nebulin appeared in an embryonic stage, and remained unchanged in molecular types throughout the entire process of development. alpha-Connectin and nebulin seemed to be regulated by a similar mechanism during development. On the other hand, beta-connectin appeared in an earlier stage of development of the embryonic breast muscle, independently of alpha-connectin.