Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Does peroxynitrite generate hydroxyl radical?

S Pou1, S Y Nguyen, T Gladwell

  • 1Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, Baltimore 21201, USA.

Biochimica Et Biophysica Acta
|May 11, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In vitro toxicity in long-term cell culture of MR contrast agents targeted to cartilage evaluation.

Osteoarthritis and cartilage·2014
Same author

Unique pharmacological actions of atypical neuroleptic quetiapine: possible role in cell cycle/fate control.

Translational psychiatry·2013
Same author

Targeted dendrimer-based contrast agents for articular cartilage assessment by MR imaging.

Osteoarthritis and cartilage·2008
Same author

Objective structured clinical examinations (OSCE) for sleep.

Sleep medicine·2005
Same author

Effects of activation of polymorphonuclear leukocytes on airway goblet cell mucin release in a co-culture system.

Inflammation research : official journal of the European Histamine Research Society ... [et al.]·2003
Same author

The single-dose pharmacokinetics of midazolam and its primary metabolite in pediatric patients after oral and intravenous administration.

Journal of clinical pharmacology·2002
Same journal

Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Molecular Basis of Disease Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

General Subjects Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Erratum to 'on the role of exchangeable hydrogen bonds for the kinetics of P680<sup>+·</sup> Q<sub>A</sub> <sup>-·</sup> formation and P680<sup>+·</sup> Pheo<sup>-·</sup> recombination in photosystem II' [Biochim. Biophys. Acta 1276 (1996) 35-44].

Biochimica et biophysica acta·2019
Same journal

Oligomeric state of the light-harvesting complexes B800-850 and B875 from purple bacterium Rubrivivax gelatinosus in detergent solution.

Biochimica et biophysica acta·2019
Same journal

Regulation of pigment content and enzyme activity in the cyanobacterium Nostoc sp. Mac grown in continuous light, a light-dark photoperiod, or darkness.

Biochimica et biophysica acta·2019
See all related articles

Peroxynitrite decomposition can produce hydroxyl radical, but at very low yields (1-4%). This suggests that hydroxyl radical is unlikely to be the primary cause of peroxynitrite-mediated cytotoxicity.

Area of Science:

  • Biochemistry
  • Free Radical Chemistry

Background:

  • Nitric oxide and superoxide react to form peroxynitrite.
  • Controversy exists regarding whether peroxynitrite decomposition generates hydroxyl radical.

Purpose of the Study:

  • To investigate the fate of peroxynitrite decomposition.
  • To determine if hydroxyl radical is produced and quantify its yield.

Main Methods:

  • Electron spin resonance (EPR) spectroscopy.
  • Spin trapping technique using 4-pyridyl 1-oxide N-tert-butylnitrone (4-POBN)/ethanol.

Main Results:

  • Peroxynitrite decomposition produces hydroxyl radical at physiological pH.
  • Hydroxyl radical yield was quantified at 1-4% in the presence or absence of chelated iron.

Related Experiment Videos

  • The low yield suggests it may not be the main contributor to cytotoxicity.

    • Conclusions:

    • Peroxynitrite decomposition does generate hydroxyl radical.
    • The low concentration of hydroxyl radical produced indicates it is not the primary mediator of peroxynitrite cytotoxicity.