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Cellular plasma membrane domains

M P Sheetz1

  • 1Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA.

Molecular Membrane Biology
|January 1, 1995
PubMed
Summary
This summary is machine-generated.

Cell migration involves distinct plasma membrane domains crucial for cell movement. These specialized membrane regions, identified using laser tweezers, guide cell direction and attachment during migration.

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Area of Science:

  • Cell Biology
  • Biophysics

Background:

  • Cell migration is a fundamental biological process.
  • The plasma membrane's role in cell migration is complex and not fully understood.
  • Specific membrane domains may regulate cell movement.

Purpose of the Study:

  • To investigate the existence and function of distinct plasma membrane domains in migrating cells.
  • To quantify the differences between these membrane domains.
  • To understand how these domains contribute to cell migration fidelity.

Main Methods:

  • Utilized laser tweezers to define membrane domains.
  • Employed single particle tracking and video analysis.
  • Studied the motile behavior of particles bound to membrane glycoproteins.

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Main Results:

  • Identified at least three distinct plasma membrane domains in migrating cells.
  • Quantified significant differences (4-20 fold) in domain properties.
  • Localized specific domains to the leading edge, rear, and curved regions of the cell.

Conclusions:

  • Plasma membrane domains are critical for cell migration.
  • These domains facilitate cytoskeletal attachment, substrata release, and membrane extension/attachment control.
  • Domain-specific properties enhance cell chemotaxis and neuronal pathfinding fidelity.