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Herpes virus-based vectors
1Department of Pathology, University of Cambridge, UK.
British Medical Bulletin
|January 1, 1995
Summary
Herpes simplex virus (HSV) shows potential as a gene delivery vehicle for lifelong latent infections. Further research in animal models is needed to define long-term gene expression from latent HSV genomes in various cell types.
Area of Science:
- Virology
- Gene Therapy
- Neuroscience
Background:
- Herpesviruses are large DNA viruses capable of lifelong latent infections.
- Herpes simplex virus (HSV) is a potential gene delivery vehicle due to its natural latency in neurons.
- Current research has primarily focused on HSV for gene delivery applications.
Purpose of the Study:
- To explore the potential of herpesviruses, particularly HSV, as gene delivery vehicles.
- To review the current state of HSV-mediated gene delivery in vitro and in vivo.
- To identify future research directions for HSV-based gene therapy.
Main Methods:
- Review of existing literature on herpesvirus and HSV gene delivery.
- Analysis of studies demonstrating reporter gene delivery using HSV vectors (replication competent and defective).
- Examination of research on physiological modifications in the central nervous system (CNS) via HSV gene delivery.
Main Results:
- HSV has been successfully used to deliver reporter genes both in vitro and in vivo.
- Significant physiological modifications in the CNS have been achieved using HSV-mediated gene delivery.
- Replication competent and defective HSV vectors have been employed for gene delivery.
Conclusions:
- HSV holds promise as a gene delivery vector for establishing latent infections.
- Further investigation using animal models is crucial to optimize HSV-mediated gene therapy.
- Defining the requirements for long-term gene expression from latent HSV genomes in diverse cell types in vivo is essential.