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[Bacterial resistance in clinical isolates of Klebsiella pneumoniae]
Abstract:
Ninety-four percent of 336 strains of Klebsiella pneumoniae were resistant to ampicillin, while less than 10% were resistant to cefotaxine and ceftazidine. Among all the aminoglycosides tested, amikacin had the strongest activity on K. pneumoniae. The resistant rate of nosocomial strains was higher than that of community strains. The results of susceptibility test showed that 42% (119/280) of K. pneumoniae strains were gentamicin-resistant. Based on the resistant pattern of K. pneumoniae, the aminoglycoside-modifying enzymes produced by the organisms were inferred. Among them 90% of 119 strains produced aminoglycosides-modifying enzymes AAC (3)-V, 8% (10/119) AAC (3)-V together with AAC (6')-Ib, AAC (6')-V or ANT (2"). The resistance of two strains was assumed to be permeability change. Besides, 58% (161/280) were sensitive strains without producing any aminoglycoside-modifying enzymes. Bacteria typing done by phage typing or Klebcin in 27 strains showed that most strains were from different origins with few exceptions. The results show the significance for controlling the nosocomial infections and reasonable use of antibiotics.
Insights
Klebsiella pneumoniae shows high ampicillin resistance but remains susceptible to cefotaxime and ceftazidime. Amikacin is effective, but gentamicin resistance is common, often due to aminoglycoside-modifying enzymes.
Area of Science:
- Microbiology
- Infectious Diseases
- Antimicrobial Resistance
Background:
- Klebsiella pneumoniae is a significant opportunistic pathogen.
- Antimicrobial resistance in K. pneumoniae poses a global health threat.
- Understanding resistance mechanisms is crucial for effective treatment.
Purpose of the Study:
- To investigate the antimicrobial susceptibility patterns of Klebsiella pneumoniae strains.
- To identify the prevalence and types of aminoglycoside-modifying enzymes.
- To differentiate resistance between nosocomial and community-acquired strains.
Main Methods:
- Antimicrobial susceptibility testing (AST) was performed on 336 Klebsiella pneumoniae strains.
- Aminoglycoside-modifying enzyme production was inferred from resistance patterns.
- Bacterial typing was conducted using phage typing and Klebcin.
Main Results:
- 94% of strains were resistant to ampicillin; <10% resistant to cefotaxime and ceftazidime.
- Amikacin showed the strongest activity among tested aminoglycosides.
- 42% of strains were gentamicin-resistant, primarily due to AAC(3)-V enzymes.
Conclusions:
- Nosocomial Klebsiella pneumoniae strains exhibit higher resistance rates than community strains.
- The prevalence of specific aminoglycoside-modifying enzymes highlights the need for targeted antibiotic use.
- Effective control of nosocomial infections requires monitoring resistance and judicious antibiotic stewardship.