Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Function of antimicrobial proteins in insects

S Natori1

  • 1Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.

Ciba Foundation Symposium
|January 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Meeting report.

Mycotoxin research·2013
Same author

The impairing effects of chaetochromin D on mitochondrial respiration and structure.

Mycotoxin research·2013
Same author

Functional flexibility of the honey bee hypopharyngeal gland in a dequeened colony.

Zoological science·2008
Same author

Identification of a novel gene, Mblk-1, that encodes a putative transcription factor expressed preferentially in the large-type Kenyon cells of the honeybee brain.

Insect molecular biology·2002
Same author

Effects of angiotensin converting enzyme inhibitor and angiotensin II type 1 receptor blocker on cardiac dysfunction induced by isoproterenol in dogs.

Journal of cardiovascular pharmacology·2002
Same author

Targeted disruption of a pupal hemocyte protein of Sarcophaga by RNA interference.

European journal of biochemistry·2001

Flesh fly larvae produce antimicrobial proteins like sarcotoxin I and sapecin to combat bacterial infections. These proteins, along with antifungal protein (AFP), protect the insect by interacting with microbial membranes.

Area of Science:

  • Insect immunology
  • Biochemistry
  • Molecular biology

Background:

  • The haemolymph of Sarcophaga peregrina larvae contains various antimicrobial proteins.
  • Sarcotoxin I targets Gram-negative bacteria, while sapecin is effective against Gram-positive bacteria.
  • Sapecin also influences insect development.

Purpose of the Study:

  • To isolate and characterize antimicrobial proteins from Sarcophaga peregrina larvae.
  • To understand the roles and mechanisms of these proteins in insect defense.

Main Methods:

  • Isolation and characterization of antimicrobial proteins from larval haemolymph.
  • Analysis of antibacterial and antifungal activities.
  • Investigation of protein interactions with microbial membranes.

Related Experiment Videos

Main Results:

  • Sarcotoxin I and sapecin are inducible proteins synthesized during bacterial infection.
  • Antifungal protein (AFP) is constitutively present and active against fungi.
  • Sarcotoxin I enhances AFP's antifungal activity synergistically.
  • Sarcotoxin I disrupts bacterial membrane functions like ATP synthesis and amino acid transport.

Conclusions:

  • Sarcophaga peregrina larvae possess a robust defense system utilizing multiple antimicrobial proteins.
  • These proteins, including sarcotoxin I, sapecin, and AFP, work together to protect against diverse microbial threats.
  • The study highlights the complex interplay of antimicrobial proteins in insect immunity.