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Related Experiment Videos

Alpha 1-adrenoceptor responsiveness in the aging aorta

H Gurdal1, G Cai, M D Johnson

  • 1Department of Pharmacology, Medical College of Pennsylvania, Philadelphia 19129, USA.

European Journal of Pharmacology
|February 14, 1995
PubMed
Summary

Aortic alpha-1 adrenoceptor subtypes change with age. Norepinephrine

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Area of Science:

  • Pharmacology
  • Cardiovascular Physiology
  • Aging Research

Background:

  • Aortic alpha-1 adrenoceptor responsiveness changes during maturation and aging.
  • Understanding changes in alpha-1 adrenoceptor subtypes is crucial for explaining altered vascular function with age.

Purpose of the Study:

  • To investigate alterations in aortic alpha-1 adrenoceptor subtypes during rat maturation and aging.
  • To determine the specific subtypes involved in age-related changes in aortic responsiveness.

Main Methods:

  • Receptor inactivation using phenoxybenzamine to assess norepinephrine affinity.
  • Selective antagonism with chlorethylclonidine to probe receptor subtypes.
  • Measurement of norepinephrine-stimulated contraction and inositol phosphate accumulation.

Main Results:

  • Apparent affinity of norepinephrine was higher in young (1-month) vs. aged (6- and 24-month) rats.
  • Chlorethylclonidine's inhibitory effect on contraction and inositol phosphate accumulation was greater in young rats.
  • Aged aortas showed partial inhibition by chlorethylclonidine, suggesting a role for a less sensitive subtype.

Conclusions:

  • Aortic alpha-1 adrenoceptor-mediated responses are primarily mediated by chlorethylclonidine-sensitive subtypes in young rats.
  • Aging is associated with an increased contribution of chlorethylclonidine-insensitive alpha-1 adrenoceptor subtypes in the aorta.

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