Related Experiment Videos
Interleukin-5 and eosinophil cationic protein in nasal lavages of rhinitis patients
I M Garrelds1, T De Graaf-in 't Veld, M A Nahori
1Department of Pharmacology, Faculty of Medicine, Erasmus University Rotterdam, Netherlands.
Abstract:
The production of interleukin-5 and eosinophil cationic protein (ECP) in the nasal cavity was examined in 24 patients with rhinitis who were allergic to the house dust mite. During a double-blind placebo-controlled cross-over study, fluticasone propionate aqueous nasal spray (200 micrograms) was administered twice daily for 2 weeks. After four basal nasal lavages provocation with house dust mite extract was performed and nasal lavages were collected every hour for 9.5 h. Interleukin-5 was present in detectable amounts in nasal lavages from patients allergic to house dust mite. Nasal challenge with house dust mite extract caused immediate nasal symptoms and increased levels of interleukin-5. Between 3.5 and 8.5 h after the challenge symptoms recurred and interleukin-5 levels increased, reflecting a late phase reaction. Eosinophil cationic protein, a marker of activated eosinophils, was released between 6.5 and 9.5 h after challenge. Treatment with fluticasone propionate (as an aqueous nasal spray) significantly decreased the evoked interleukin-5 and ECP levels in the late phase reaction. This response was correlated with an improved symptom score. This could indicate that the number and activity of eosinophils are increased during the late phase allergic reaction, a response that is inhibited by corticosteroids.
Insights
Fluticasone propionate nasal spray reduced interleukin-5 and eosinophil cationic protein (ECP) levels in patients with house dust mite allergy. This treatment improved late-phase allergic reaction symptoms.
Area of Science:
- Immunology
- Allergology
- Pharmacology
Background:
- Rhinitis involves nasal cavity inflammation.
- Allergic rhinitis is often triggered by house dust mites.
- Interleukin-5 and ECP are key inflammatory markers.
Purpose of the Study:
- To investigate the effect of fluticasone propionate on interleukin-5 and ECP production in house dust mite-allergic rhinitis.
- To assess the role of these mediators in the late-phase allergic reaction.
Main Methods:
- Double-blind, placebo-controlled crossover study.
- 24 patients with house dust mite allergy received fluticasone propionate nasal spray or placebo.
- Nasal lavages were collected after allergen challenge to measure interleukin-5 and ECP.
Main Results:
- House dust mite challenge increased interleukin-5 levels, indicating an allergic response.
- Interleukin-5 and ECP were elevated during the late-phase reaction.
- Fluticasone propionate significantly reduced interleukin-5 and ECP levels in the late phase.
- Treatment correlated with improved symptom scores.
Conclusions:
- Corticosteroids like fluticasone propionate inhibit eosinophil activity in allergic rhinitis.
- The late-phase allergic reaction involves increased eosinophil numbers and activity.
- Fluticasone propionate is effective in managing house dust mite-induced allergic rhinitis.